LIVER AND ADIPOSE TISSUES OF MICE CONCEIVED BY IN VITRO FERTILIZATION (IVF) SHOW INCREASED OXIDATIVE DAMAGE

Some human and animal studies indicate that adult offspring conceived by IVF show a predisposition to glucose intolerance hypertension. At present, there is not molecular mechanism can explain these findings. Since oxidative stress has been linked diabetes hypertension since generated embryos are known be exposed high levels of reactive oxygen species (ROS), this study was performed understand if damage present in liver adipose tissue mouse IVF. Experimental study. CF1 x B6D2F1 mice were using potassium-enriched simplex optimized medium (KSOM) 5% O2. Blastocysts natural mating (flushed blastocysts = FB group) transferred recipients used as control. IVF, embryo culture transfer at the blastocyst stage routinely done lab. 40 weeks life, sacrificed, gonadal harvested, frozen stored -80 Celsius. Tissues assessed for glutathione (GSH) DNA (by measuring 8-hydroxy-deoxyguanosine- 8-OHdG) proteins (carbonyl-group) available kits. Lipids (F2α isoprostane) mass spectrometry. Parametric statistics p value < 0.05 considered significant. Tabled 1IVF (n=11)FB (n=10)p value8-OH-dG (ng/ml)11.1810.610.048-OH-dG fat (ng/ml)11.0710.40NSF2α-isoprostane (ng/ml)18.9714.210.08F2α-isoprostane (ng/ml)8.874.520.04Carbonyl (nmol/mg)1.161.820.07Carbonyl (nmol/mg)2.121.85NSGlutathione (μM)0.080.05NSGlutathione (μM)0.020.010.01NS Not Significant Open table new tab NS Liver IVF-generated biochemical evidence persistent altered antioxidant levels. These results might suggest link between metabolic dysfunction found mice. Further needed mechanism(s) leading increase discover strategies mitigate differences.